Data analysis Essay Example | Topics and Well Written Essays - 500 words

information analysis - Essay ExampleThe Experiment of Western blotting analysis for the samples of NIH/3T3 cells, whether having been treated in PDGF or not, uses the Phospho-Akt (Ser473) for the expression of the upper blotting and Akt 9272 for the lower blotting. It has to present the results in a tabular form, the first column being PGDF, the s being AKT and the third column being Phospho-Akt.This was a time series practical activity to find the cells at various durations from 0 to 120 minutes. Its independent variable was the PGDF. There ar two blots to analyze, Ser473 of Akt as the upper blot (using phospho-Akt antibody) and phosphor-Akt (using Akt antibody) as the lower blot and. It also involved using gels to conduct the molecular weight marker. The top band corresponded to the measurement of 76 kDa, while the middle band corresponded to 57 kDa. The concluding band thus corresponded to 47 kDa.The growth factor analysis shows that proteins facilitate growth at dissimila r rates under different conditions. The most relevant protein analysis procedure in this kind of experimentation is the florescent microscopy.From the principles of cytometry, this experiment conducts the flow cytometry experiment with the labeling of Jurkat cells and the labeling of Phospho-Akt ser 473antibody. It uses three categories of cells, untreated, treated cells and control experiment (Burgering and Coffer 1995, p54). All the cells are labeled in three different colors as they appear in the graph above. The crimson line is the control experiment, green is treated and blue is